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There are various cannabinoids manufactured from the hemp or cannabis plant. The two most abundant ones are cannabidiol (CBD) and Tetrahydrocannabinol (THC) which are widely used for their medicinal effects and has been significantly studied by researchers. However, the focus has shifted from CBD and THC to other cannabinoids.

CBN is another form of cannabinoid which has started to gain the attention of users and researchers. Its purest form is known as CBN isolate. Just like other cannabinoids, it also has medicinal benefits and therapeutic uses. Have you ever wondered how to make CBN isolate?


CBN, also known as Cannabinol, is one of the hundreds of cannabinoids obtained from the hemp plant. Its concentration increases as the cannabis plant ages; this effect occurs due to the light and oxygen that acts on the cannabis plant, which causes its THC to degrade into CBN. This action happens naturally but can also be stimulated in the lab by making use of heat and oxidation.

In 1896, CBN became the first cannabinoid to be extracted from cannabis. What led to the extraction was the keen interest of researchers to find out the primary compound responsible for the psychoactive effect of marijuana, and CBN was thought to be it, but it wasn’t. It was later discovered that THC is the primary psychoactive compound, CBN is a by-product of THC, and it’s only capable of producing light intoxicating effects.

The THC in the hemp plant that decomposes into CBN is never found in quantities greater than 0.3%. Cannabinol is not easily found because of the difficulty accompanying its production. It’s found only in minute amounts across most commercially grown strains.

However, cannabis that’s mature or well-aged displays a high level of CBN, which makes it a better option for researchers when carrying out experiments. Although it’s a challenging production method, its benefits make the process worthwhile.


There are many forms of CBN extracts available in the market, the most popular one is the CBN isolate which is sometimes referred to as distillate, it’s the pure form of CBN compound. The isolation process aims to remove all other forms of secondary components present in CBN such as other cannabinoids, terpenoids, flavortipends and inactive compounds- to create a final product devoid of odor and flavor with a CBN concentration that exceeds 99 per cent.


THC degrades into CBN as the plant ages, a freshly budded hemp plant contains only 1% of Cannabinol in its entire profile. In contrast, a cannabis plant that’s matured or well-aged contains a high amount of Cannabinol and it’s used in the extraction of CBN isolate. There are many extraction methods used across the cannabis industry to produce CBN isolate but the two common and preferred ones are solvent extraction and CO2 extraction.

  1. Solvent extraction: Just like the name suggests, this method involves the use of solvents to obtain CBN isolate. Solvents such as ethanol are applied to the cannabis or hemp plant to filter out other cannabinoids. This method is relatively cheaper, but at the same time, it has a significant disadvantage. During the extraction of CBN isolate, an undesirable chlorophyll is extracted along the process, it has a bitter taste and is green in color, this calls for a different refinement process.
  2. C02 extraction: This is the method manufacturers of mid-to-high-end products prefer. The industrial-grade closed loop system cools and pressures C02 into a “Supercritical” state. While in this simultaneous liquid and gaseous state, it’s used on the cannabis or hemp plant (similar to solvent) to extract cannabinoids and terpenes without extracting chlorophyll alongside it. Even though C02 extraction is way more expensive than other extraction methods, it’s currently the industry’s most recognized method for manufacturing cannabinoid isolates.After the isolation process is completed, the CBN isolate obtains a high level of concentration and becomes tasteless, this makes it versatile and suitable for use across a wide range of applications. If the production is carried out according to the industry production standard, an exact yield of 1mg of CBN per milligram would be obtained. This enables it to be easily used for infusion into different products such as balms, creams, medicine and even beauty supplies.Meanwhile, a lower-quality isolate always falls between 0.50-0.70 mg of CBN per milligram. This makes it a bit more challenging to use compared to industry standard isolates with a 1:1 ratio of concentration. These products can be easily identified with a compliant-certification label in their package, and they are in higher demand than others.


Cannabinol (CBN) is difficult to obtain and it’s not found in significant quantities because it’s a minor cannabinoid. Because of this reason, CBN extract is hard to find. However, CBN isolate can also be synthetically produced in the lab. But the lab-made cannabinoids including synthetic CBN isolate, are banned in the United States.
Sadly, many people are falling victim to retailers of synthetic cannabinoids who portray their products as ‘natural’ or as a means of experiencing a feeling of being high, which THC causes. Most times, the synthetic cannabinoids manufactured are not from plants; no reason or proof shows that synthetic cannabinoids produce therapeutic benefits.
The food and drug administration (FDA) has not evaluated and approved synthetic cannabinoids for safe use. Synthetic cannabinoids also produce adverse reactions in some people when consumed, which can be deadly and life-threatening.

Description: Cannabinoid Extract


CBN isolate is the pure form of CBN, purified from all other components such as other cannabinoids, terpenoids and others. It also contains a very high level of CBN. These qualities cause it to be helpful in vast areas of applications. However, some questions tend to arise concerning its benefits, and some of them are:


Many manufacturers advertise CBN as a sleep-supporting substance, but there’s no proof or research to back up their claims.

The prominent argument people use to support this speculation is that when the cannabis plant is left exposed to oxygen and heat, THC degrades into CBN, and as this reaction continues, CBN starts to lose its psychoactivity and the sedative potency begins to increase. This inverse relationship between the level of THC and CBN has made many cannabis growers and enthusiasts believe that CBN is the primary component that gives the cannabis plant its sedative potency.

However, there’s no evidence to support this belief, and the existing CBN research didn’t present CBN as a sleep-supporting system. In 1975, a study revealed that CBN isolate didn’t contain sedative properties. The study was conducted with human subjects and tested with CBN, THC and a combination of both in three different experimental periods.

CBN didn’t exhibit any effect on its own. In the case of THC, a drowsy effect was observed, and a more sedative effect was produced when the two were used together. The researcher concluded that CBN has little sedative properties, but understanding its use with THC would require more research.

Dr Ethan Russo, a prominent cannabis researcher, also argued that CBN doesn’t have a powerful sedative potency. It can only produce sedative effects when combined with THC and CBD. This renders using CBN isolates powder or oil to provide supporting qualities for sleep invalid.


Research has proven THC to be the significant psychoactive compound present in marijuana that’s responsible for its intoxicating effects. Chemically, the structure of THC resembles the compound our body produces naturally, called Anandamide or the ‘bliss molecule.’

Both Anandamide and THC cause the release of an enzyme called serotonin by binding themselves to the CB1 receptor in the brain. The enzyme released affects the sense of well-being and distorts perception. CBN also produces a similar effect because it’s a by-product of THC and can bind to the CB1 receptor. However, its effects are much lighter.

When CBN is used with THC, it helps improve THC’s overall effect, especially in appetite stimulation and intoxication. But CBN alone doesn’t tend to bind with CB1 receptors. Therefore it’s not considered to be much of an intoxicating component. It can produce light intoxicating effects if used in a huge amount. But people don’t turn to CBN isolate when they desire to get high because of its high price.

Description: Cannabis leaves oil extract


This thought came into existence when Weydt et al. succeeded in a study involving the use of CBN to treat mice with amyotrophic lateral sclerosis(ACS). The symptom of the disorder was successfully reduced, and the onset of it was delayed.

These results have led to speculations that CBN can be used to achieve healthy brain function and to treat neurodegenerative diseases. CBN isolate may be used to maintain a mental focus. However, it’s advisable to combine it with other compounds. If you want a healthy brain function, then the best option is to combine CBN with other cannabinoids that support mental clarity.


CBN can inhibit the enzymes that activate inflammation to support a healthy inflammatory response. This can be useful in healthy joint mobility and an average post-walk recovery. Another option to maintain healthy mobility is to take Cannabinol with compounds with similar benefits, such as CBD. CBD has been significantly researched for its healing, mobility and recovery benefits.


In 2008, a laboratory test was done by Appendicoe et al., which revealed that CBN could be used as an effective antibacterial agent, especially against antibiotic-resistant strains of MRSA bacteria. With more and more experiments being conducted, CBN might later have its place in the future of antibiotics.


There have been a lot of discoveries made on hemp plants over the past years, various cannabinoids are being discovered, and intense research is being conducted to determine the potential health benefits of CBR, THC, CBG, CBN and other products in the cannabinoid industry. This makes it easier for entrepreneurs to devise trickery methods to sell their products.

However, the good news is that you can always know how to avoid buying a wrong or fake product thanks to the Food and Drug Administration (FDA), which has placed some strict marketing restrictions on hemp products to protect the customers. It’s important to note that no brand or company can ethically claim to diagnose, treat, cure or prevent should be avoided.

To secure a good CBN isolate, ensure you conduct proper research before setting out to buy. This will allow you to identify false claims made by brands and prevent you from buying CBN isolate-related- products that their uses are not based on scientific evidence.


CBN isolate is a pure form of CBN, making it suitable for anyone looking to take in the full benefits of CBN. It can be used for a wide range of products such as concentrates, topicals and tinctures. Some examples of health supplements that are popularly infused with CBN are organic tea products, diffuser oil and full-spectrum CBD.

Like CBD, Cannabinol effortlessly mixes with a carrier oil, making it easy to use as part of your recipes. People often prefer homemade CBN oil because it allows you to make the oil as you deem fit and tailor it to meet your needs.


There are various types of cannabinoids obtained from the Hemp plant, one of which is CBN which is gaining attention from users and researchers alike. It’s a by-product of THC, and its concentration level increases as the plant ages. Its pure form is known as CBN isolate. Many methods can be used to obtain CBN isolate, but the two commonly used ones are solvent extraction and CO2 extraction.

CBN isolate can also be manufactured synthetically. However, its synthetic CBN is unsuitable for consumption as it produces life-threatening reactions when taken by certain people. There are also various misconceptions people have about CBN concerning its sedative potency, intoxicating properties, and mental focus—CBN isolate can help achieve all this mildly. It’s more effective when used in combination with other THC and cannabinoids.

As more research is done on CBN isolate, we’ll continue to witness its use in areas such as antibacterial. The nature of CBN isolates makes it easy to be infused into a wide range of products. Before setting out to buy CBN isolate, ensure you conduct good research so as not to fall victim to fake products. Lastly, CBN isolate is pure and highly recommended to anyone who wants to take in the full benefits CBN has to Offer.